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oxiselect age competitive elisa kit  (Cell Biolabs Inc)

 
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    Cell Biolabs Inc oxiselect age competitive elisa kit
    Glycogen and trehalose synergistically buffer against glucose stress. ( A ) Glycogen and trehalose quantification of WT stationary phase cultures (16 h post-inoculation) in LB medium and LB medium supplemented with increasing glucose concentrations (0.1%, 0.2%, and 0.4%). Data are mean ± s.d.( n = 3 cultures per strain or per condition. Statistical significance of glucose-supplemented conditions is compared to LB-only media condition: *, P < 0.05; **, P <0.01 ; ***, P < 0.001; unpaired t -test; two-sided). ( B ) Growth curve of strains in LB + 0.4% glucose. Black filled squares, WT; dark gray open squares, otsA; gray circles, glgA ; light gray triangles, glgA otsA . (↓= below limit of detection, 10 3 CFU/mL) Statistically significant timepoints are indicated in the accompanying table: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant; unpaired t-test; two-sided. ( C ) <t>AGE</t> <t>ELISA.</t> Data are mean ± s.d. ( n = 3 cultures per strain or per condition; Statistical significance of AGE concentration of mutant strains is compared to AGE concentration of WT for the corresponding day/condition; *, P < 0.05; unpaired t -test; two-sided).
    Oxiselect Age Competitive Elisa Kit, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oxiselect+age+competitive+elisa+kit/adduct+competitive+elisa+hne+kit+oxiselect/pmc13192265-65-24-29
    Average 86 stars, based on 1 article reviews
    oxiselect age competitive elisa kit - by Bioz Stars, 2026-09
    86/100 stars

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    1) Product Images from "Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli"

    Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli

    Journal: Journal of Bacteriology

    doi: 10.1128/jb.00544-25

    Glycogen and trehalose synergistically buffer against glucose stress. ( A ) Glycogen and trehalose quantification of WT stationary phase cultures (16 h post-inoculation) in LB medium and LB medium supplemented with increasing glucose concentrations (0.1%, 0.2%, and 0.4%). Data are mean ± s.d.( n = 3 cultures per strain or per condition. Statistical significance of glucose-supplemented conditions is compared to LB-only media condition: *, P < 0.05; **, P <0.01 ; ***, P < 0.001; unpaired t -test; two-sided). ( B ) Growth curve of strains in LB + 0.4% glucose. Black filled squares, WT; dark gray open squares, otsA; gray circles, glgA ; light gray triangles, glgA otsA . (↓= below limit of detection, 10 3 CFU/mL) Statistically significant timepoints are indicated in the accompanying table: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant; unpaired t-test; two-sided. ( C ) AGE ELISA. Data are mean ± s.d. ( n = 3 cultures per strain or per condition; Statistical significance of AGE concentration of mutant strains is compared to AGE concentration of WT for the corresponding day/condition; *, P < 0.05; unpaired t -test; two-sided).
    Figure Legend Snippet: Glycogen and trehalose synergistically buffer against glucose stress. ( A ) Glycogen and trehalose quantification of WT stationary phase cultures (16 h post-inoculation) in LB medium and LB medium supplemented with increasing glucose concentrations (0.1%, 0.2%, and 0.4%). Data are mean ± s.d.( n = 3 cultures per strain or per condition. Statistical significance of glucose-supplemented conditions is compared to LB-only media condition: *, P < 0.05; **, P <0.01 ; ***, P < 0.001; unpaired t -test; two-sided). ( B ) Growth curve of strains in LB + 0.4% glucose. Black filled squares, WT; dark gray open squares, otsA; gray circles, glgA ; light gray triangles, glgA otsA . (↓= below limit of detection, 10 3 CFU/mL) Statistically significant timepoints are indicated in the accompanying table: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant; unpaired t-test; two-sided. ( C ) AGE ELISA. Data are mean ± s.d. ( n = 3 cultures per strain or per condition; Statistical significance of AGE concentration of mutant strains is compared to AGE concentration of WT for the corresponding day/condition; *, P < 0.05; unpaired t -test; two-sided).

    Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay, Mutagenesis

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    Article Snippet: .. Serum concentrations of advanced glycation-end products (AGEs), soluble receptor of AGEs (sRAGE), growth differentiation factor 9 (GDF9), and bone morphogenetic protein 15 (BMP15) were measured using an OxiSelect AGE Competitive ELISA Kit (Cell BioLabs, Inc., San Diego, CA, USA), human RAGE Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), Human GDF9 ELISA Kit (LifeSpan BioSciences, Inc., Lynnwood, WA, USA), and Human BMP15 ELISA Kit (CUSABIO, Houston, TX, USA), respectively. .. Oxidative stress and antioxidant potential were measured using a Diacron reactive oxygen metabolites (d-ROMs)/biological antioxidant potential (BAP) Kit and Free Radical Elective Evaluator (FREE Carrio Duo; Diacron International srl, Grosseto, Italy).

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    Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli
    Article Snippet: Cells were sonicated on ice using a Branson Sonifier 250 (Emerson) set to maximum setting, six times for 10 s with 30-s rest, followed by enzymatic digestion with DNase I (50 μg/mL), RNase A (50 μg/mL), and lysozyme (20 μg/mL) at 37°C for 1 h. Protein concentration of the clarified lysate was quantified using the Quick Start Bradford dye reagent, following the manufacturer’s instructions (Bio-Rad). .. 100 μL of protein extract, at concentrations ranging from 0.5 to 1.0 mg/mL, was added to an AGE conjugate-coated plate as part of an OxiSelect AGE Competitive ELISA Kit (Cell Biolabs). ..

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    Article Snippet: .. Monitoring of Advanced Glycation End Products (AGEs) levels was assessed similarly to PCC with the OxiSelect AGE Competitive ELISA Kit (Cell Biolabs). ..

    Article Title: Attenuation of Chronic Stress-Induced Depressive-like Symptoms by Fish Oil via Alleviating Neuroinflammation and Impaired Tryptophan Metabolism in Aging Rats.
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    Article Snippet: .. The AGE levels were monitored in a similar manner to PCC using an OxiSelect AGE competitive ELISA Kit (Cell Biolabs, San Diego, CA, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Assessment and prediction models for the quantitative and qualitative reserve of the ovary using machine learning
    Article Snippet: .. Serum concentrations of advanced glycation-end products (AGEs), soluble receptor of AGEs (sRAGE), growth differentiation factor 9 (GDF9), and bone morphogenetic protein 15 (BMP15) were measured using an OxiSelect AGE Competitive ELISA Kit (Cell BioLabs, Inc., San Diego, CA, USA), human RAGE Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), Human GDF9 ELISA Kit (LifeSpan BioSciences, Inc., Lynnwood, WA, USA), and Human BMP15 ELISA Kit (CUSABIO, Houston, TX, USA), respectively. .. Oxidative stress and antioxidant potential were measured using a Diacron reactive oxygen metabolites (d-ROMs)/biological antioxidant potential (BAP) Kit and Free Radical Elective Evaluator (FREE Carrio Duo; Diacron International srl, Grosseto, Italy).

    Article Title: Attenuation of Chronic Stress-Induced Depressive-like Symptoms by Fish Oil via Alleviating Neuroinflammation and Impaired Tryptophan Metabolism in Aging Rats
    Article Snippet: .. Plasma 8-OHdG was examined using a DNA/RNA oxidative damage enzyme-linked immunosorbent assay (ELISA) kit (589320, Cayman Chemical, Ann Arbor, MI, USA), plasma AGEs were examined using the OxiSelect AGE Competitive ELISA kit (STA-817, Cell Biolabs, San Diego, CA, USA), and MDA was examined using colorimetric 2-thiobarbituric acid reactive substances (TBARS) assay. .. The hippocampus TNF-α level was examined using a rat TNF-α ELISA kit (438207, BioLegend, San Diego, CA, USA) in accordance with the manufacturer’s instructions.

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    Periodic Counter-current Chromatography:

    Article Title: Antioxidant Potential and Oxidative Stress Modulation of Geranium macrorrhizum L. Oil Extract in Gentamicin-Induced Nephrotoxicity
    Article Snippet: .. The AGE levels were monitored in a similar manner to PCC using an OxiSelect AGE competitive ELISA Kit (Cell Biolabs, San Diego, CA, USA). ..

    Article Title: Pulmonary Protein Oxidation and Oxidative Stress Modulation by Lemna minor L. in Progressive Bleomycin-Induced Idiopathic Pulmonary Fibrosis
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    Clinical Proteomics:

    Article Title: Attenuation of Chronic Stress-Induced Depressive-like Symptoms by Fish Oil via Alleviating Neuroinflammation and Impaired Tryptophan Metabolism in Aging Rats
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    Article Title: Attenuation of Chronic Stress-Induced Depressive-like Symptoms by Fish Oil via Alleviating Neuroinflammation and Impaired Tryptophan Metabolism in Aging Rats.
    Article Snippet: The prevalence of depression is increasing, and geriatric depression, in particular, is difficult to recognize and treat.. Depression in older adults is often accompanied by neuroinflammation in the central nervous system (CNS).. Neuroinflammation affects the brain’s physiological and immune functions through several pathways and induces depressive symptoms.

    Multiple Displacement Amplification:

    Article Title: Attenuation of Chronic Stress-Induced Depressive-like Symptoms by Fish Oil via Alleviating Neuroinflammation and Impaired Tryptophan Metabolism in Aging Rats
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    Article Title: Attenuation of Chronic Stress-Induced Depressive-like Symptoms by Fish Oil via Alleviating Neuroinflammation and Impaired Tryptophan Metabolism in Aging Rats.
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    TBARS Assay:

    Article Title: Attenuation of Chronic Stress-Induced Depressive-like Symptoms by Fish Oil via Alleviating Neuroinflammation and Impaired Tryptophan Metabolism in Aging Rats
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    Article Snippet: The prevalence of depression is increasing, and geriatric depression, in particular, is difficult to recognize and treat.. Depression in older adults is often accompanied by neuroinflammation in the central nervous system (CNS).. Neuroinflammation affects the brain’s physiological and immune functions through several pathways and induces depressive symptoms.



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    Glycogen and trehalose synergistically buffer against glucose stress. ( A ) Glycogen and trehalose quantification of WT stationary phase cultures (16 h post-inoculation) in LB medium and LB medium supplemented with increasing glucose concentrations (0.1%, 0.2%, and 0.4%). Data are mean ± s.d.( n = 3 cultures per strain or per condition. Statistical significance of glucose-supplemented conditions is compared to LB-only media condition: *, P < 0.05; **, P <0.01 ; ***, P < 0.001; unpaired t -test; two-sided). ( B ) Growth curve of strains in LB + 0.4% glucose. Black filled squares, WT; dark gray open squares, otsA; gray circles, glgA ; light gray triangles, glgA otsA . (↓= below limit of detection, 10 3 CFU/mL) Statistically significant timepoints are indicated in the accompanying table: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant; unpaired t-test; two-sided. ( C ) <t>AGE</t> <t>ELISA.</t> Data are mean ± s.d. ( n = 3 cultures per strain or per condition; Statistical significance of AGE concentration of mutant strains is compared to AGE concentration of WT for the corresponding day/condition; *, P < 0.05; unpaired t -test; two-sided).
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    Glycogen and trehalose synergistically buffer against glucose stress. ( A ) Glycogen and trehalose quantification of WT stationary phase cultures (16 h post-inoculation) in LB medium and LB medium supplemented with increasing glucose concentrations (0.1%, 0.2%, and 0.4%). Data are mean ± s.d.( n = 3 cultures per strain or per condition. Statistical significance of glucose-supplemented conditions is compared to LB-only media condition: *, P < 0.05; **, P <0.01 ; ***, P < 0.001; unpaired t -test; two-sided). ( B ) Growth curve of strains in LB + 0.4% glucose. Black filled squares, WT; dark gray open squares, otsA; gray circles, glgA ; light gray triangles, glgA otsA . (↓= below limit of detection, 10 3 CFU/mL) Statistically significant timepoints are indicated in the accompanying table: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant; unpaired t-test; two-sided. ( C ) AGE ELISA. Data are mean ± s.d. ( n = 3 cultures per strain or per condition; Statistical significance of AGE concentration of mutant strains is compared to AGE concentration of WT for the corresponding day/condition; *, P < 0.05; unpaired t -test; two-sided).

    Journal: Journal of Bacteriology

    Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli

    doi: 10.1128/jb.00544-25

    Figure Lengend Snippet: Glycogen and trehalose synergistically buffer against glucose stress. ( A ) Glycogen and trehalose quantification of WT stationary phase cultures (16 h post-inoculation) in LB medium and LB medium supplemented with increasing glucose concentrations (0.1%, 0.2%, and 0.4%). Data are mean ± s.d.( n = 3 cultures per strain or per condition. Statistical significance of glucose-supplemented conditions is compared to LB-only media condition: *, P < 0.05; **, P <0.01 ; ***, P < 0.001; unpaired t -test; two-sided). ( B ) Growth curve of strains in LB + 0.4% glucose. Black filled squares, WT; dark gray open squares, otsA; gray circles, glgA ; light gray triangles, glgA otsA . (↓= below limit of detection, 10 3 CFU/mL) Statistically significant timepoints are indicated in the accompanying table: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant; unpaired t-test; two-sided. ( C ) AGE ELISA. Data are mean ± s.d. ( n = 3 cultures per strain or per condition; Statistical significance of AGE concentration of mutant strains is compared to AGE concentration of WT for the corresponding day/condition; *, P < 0.05; unpaired t -test; two-sided).

    Article Snippet: 100 μL of protein extract, at concentrations ranging from 0.5 to 1.0 mg/mL, was added to an AGE conjugate-coated plate as part of an OxiSelect AGE Competitive ELISA Kit (Cell Biolabs).

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Mutagenesis

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    Figure Lengend Snippet: Effects of MOLD with LPS on AGEs and RAGE production in RAW 264.7 cells. (A) Chemical structure of MOLD. (B) Cell proliferation analysis using the CCK-8 assay in RAW 264.7 cells. (C) Analysis of AGEs production using an ELISA kit in RAW 264.7 cell lysates. (D) AGEs and RAGE protein expression confirmed by Western blot analysis in RAW 264.7 cells. β-Actin was used as an internal control. (E) IHC staining of AGEs in RAW 264.7 macrophages incubated with or without MOLD for 24 h (×400 magnification). (F) IHC staining of RAGE in macrophages incubated with or without MOLD for 24 h in RAW 264.7 cells (×400 magnification). Each data point represents the mean±standard error of the mean for n=3 experiments. * P <0.05. MOLD, methylglyoxal-lysine dimer; LPS, lipopolysaccharide; AGEs, advanced glycation end products; RAGE, receptor for AGEs; CCK-8, Cell Counting Kit-8; ELISA, enzyme-linked immunosorbent assay; IHC, immunohistochemistry.

    Article Snippet: Moreover, the cells were harvested to measure AGE concentration using the OxiSelect TM AGE Competitive ELISA Kit (Cell Biolabs Inc.) in accordance with the manufacturer’s instructions.

    Techniques: CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control, Immunohistochemistry, Incubation, Cell Counting

    Effects of MOLD with LPS on NO, PGE 2 production, and cytokine levels in RAW 264.7 cell culture media. (A) Nitrite production in RAW 264.7 cells. (B) iNOS protein expression in RAW 264.7 cells. (C) PGE 2 production in RAW 264.7 cells. (D) COX-2 protein expression in RAW 264.7 cells. (E) TNF-α levels measured by ELISA in RAW 264.7 cells. (F) IL-6 levels measured by ELISA in RAW 264.7 cells. (G) TNF-α protein expression in RAW 264.7 cells. β-Actin was used as an internal control. (H) IL-6 protein expression in RAW 264.7 cells. β-Actin was used as an internal control. Each data point represents the mean±standard error of the mean for n=3 experiments. * P <0.05. MOLD, methylglyoxal-lysine dimer; LPS, lipopolysaccharide; NO, nitric oxide; PGE 2 , prostaglandin E2; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase-2; TNF-α, tumor necrosis factor alpha; ELISA, enzyme-linked immunosorbent assay; IL-6, interleukin-6.

    Journal: Preventive Nutrition and Food Science

    Article Title: NRF2 Activation Suppressed Methylglyoxal-Lysine Dimer-Induced Oxidative Stress and Inflammatory Paracrine Interaction between Macrophages and Adipocytes

    doi: 10.3746/pnf.2025.30.5.450

    Figure Lengend Snippet: Effects of MOLD with LPS on NO, PGE 2 production, and cytokine levels in RAW 264.7 cell culture media. (A) Nitrite production in RAW 264.7 cells. (B) iNOS protein expression in RAW 264.7 cells. (C) PGE 2 production in RAW 264.7 cells. (D) COX-2 protein expression in RAW 264.7 cells. (E) TNF-α levels measured by ELISA in RAW 264.7 cells. (F) IL-6 levels measured by ELISA in RAW 264.7 cells. (G) TNF-α protein expression in RAW 264.7 cells. β-Actin was used as an internal control. (H) IL-6 protein expression in RAW 264.7 cells. β-Actin was used as an internal control. Each data point represents the mean±standard error of the mean for n=3 experiments. * P <0.05. MOLD, methylglyoxal-lysine dimer; LPS, lipopolysaccharide; NO, nitric oxide; PGE 2 , prostaglandin E2; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase-2; TNF-α, tumor necrosis factor alpha; ELISA, enzyme-linked immunosorbent assay; IL-6, interleukin-6.

    Article Snippet: Moreover, the cells were harvested to measure AGE concentration using the OxiSelect TM AGE Competitive ELISA Kit (Cell Biolabs Inc.) in accordance with the manufacturer’s instructions.

    Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay, Control

    Effects of macrophage-derived CM on 3T3-L1 cells. (A) AGEs were analyzed using an ELISA kit in 3T3-L1 cell lysates. (B) AGEs and RAGE protein expression was analyzed by Western blot analysis in 3T3-L1 cells. β-Actin was used as an internal control. (C) ORO staining for the analysis of lipid accumulation in 3T3-L1 cells (×200 magnification). (D) Cell cycle analysis in 3T3-L1 cells. Cell populations at each stage were determined using the FCS Express 4 Flow Research Edition program. (E) c/EBPβ, PPARγ, and c/EBPα protein expression in 3T3-L1 cells. β-Actin was used as an internal control. (F) TNF-α and IL-6 protein expression in 3T3-L1 cells. β-Actin was used as an internal control. Each data point represents the mean±standard error of the mean for n=3 experiments. * P <0.05. CM, conditioned medium; AGEs, advanced glycation end products; ELISA, enzyme-linked immunosorbent assay; RAGE, receptor for AGEs; ORO, Oil Red O; TNF-α, tumor necrosis factor alpha; IL-6, interleukin-6.

    Journal: Preventive Nutrition and Food Science

    Article Title: NRF2 Activation Suppressed Methylglyoxal-Lysine Dimer-Induced Oxidative Stress and Inflammatory Paracrine Interaction between Macrophages and Adipocytes

    doi: 10.3746/pnf.2025.30.5.450

    Figure Lengend Snippet: Effects of macrophage-derived CM on 3T3-L1 cells. (A) AGEs were analyzed using an ELISA kit in 3T3-L1 cell lysates. (B) AGEs and RAGE protein expression was analyzed by Western blot analysis in 3T3-L1 cells. β-Actin was used as an internal control. (C) ORO staining for the analysis of lipid accumulation in 3T3-L1 cells (×200 magnification). (D) Cell cycle analysis in 3T3-L1 cells. Cell populations at each stage were determined using the FCS Express 4 Flow Research Edition program. (E) c/EBPβ, PPARγ, and c/EBPα protein expression in 3T3-L1 cells. β-Actin was used as an internal control. (F) TNF-α and IL-6 protein expression in 3T3-L1 cells. β-Actin was used as an internal control. Each data point represents the mean±standard error of the mean for n=3 experiments. * P <0.05. CM, conditioned medium; AGEs, advanced glycation end products; ELISA, enzyme-linked immunosorbent assay; RAGE, receptor for AGEs; ORO, Oil Red O; TNF-α, tumor necrosis factor alpha; IL-6, interleukin-6.

    Article Snippet: Moreover, the cells were harvested to measure AGE concentration using the OxiSelect TM AGE Competitive ELISA Kit (Cell Biolabs Inc.) in accordance with the manufacturer’s instructions.

    Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control, Staining, Cell Cycle Assay

    Effect of NK on the elevation of AGE-albumin level in STZ-induced diabetic rats. The rats in the NK-L, NK-H, or control groups were fed low-NK diet (0.2 mg/g CE-2), high-NK diet (0.6 mg/g CE-2), and CE-2, respectively, from Day 0 to Day14 after STZ administration. Rats in the sham group (sham) were fed CE-2 for 14 days without STZ injection. As described in the Materials and Methods section, the circulating AGE-albumin levels in each group on day 14 measured using AGE-competitive ELISA kit. Data are expressed as mean ± S.E.M. # p < 0.05 versus sham (Student's t -test), * p < 0.05 versus control (Dunnett's test).

    Journal: Heliyon

    Article Title: Effect of nattokinase on the pathological conditions in streptozotocin induced diabetic rats

    doi: 10.1016/j.heliyon.2024.e28835

    Figure Lengend Snippet: Effect of NK on the elevation of AGE-albumin level in STZ-induced diabetic rats. The rats in the NK-L, NK-H, or control groups were fed low-NK diet (0.2 mg/g CE-2), high-NK diet (0.6 mg/g CE-2), and CE-2, respectively, from Day 0 to Day14 after STZ administration. Rats in the sham group (sham) were fed CE-2 for 14 days without STZ injection. As described in the Materials and Methods section, the circulating AGE-albumin levels in each group on day 14 measured using AGE-competitive ELISA kit. Data are expressed as mean ± S.E.M. # p < 0.05 versus sham (Student's t -test), * p < 0.05 versus control (Dunnett's test).

    Article Snippet: OxiSelect™ Advanced Glycation End Product (AGE) Competitive ELISA Kit was obtained from Cell Biolabs (Danvers, MA, USA).

    Techniques: Injection, Competitive ELISA