oxiselect age competitive elisa kit  (Cell Biolabs Inc)

Journal: Journal of Bacteriology

Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli

doi: 10.1128/jb.00544-25

Figure Lengend Snippet: Glycogen and trehalose synergistically buffer against glucose stress. ( A ) Glycogen and trehalose quantification of WT stationary phase cultures (16 h post-inoculation) in LB medium and LB medium supplemented with increasing glucose concentrations (0.1%, 0.2%, and 0.4%). Data are mean ± s.d.( n = 3 cultures per strain or per condition. Statistical significance of glucose-supplemented conditions is compared to LB-only media condition: *, P < 0.05; **, P <0.01 ; ***, P < 0.001; unpaired t -test; two-sided). ( B ) Growth curve of strains in LB + 0.4% glucose. Black filled squares, WT; dark gray open squares, otsA; gray circles, glgA ; light gray triangles, glgA otsA . (↓= below limit of detection, 10 3 CFU/mL) Statistically significant timepoints are indicated in the accompanying table: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant; unpaired t-test; two-sided. ( C ) AGE ELISA. Data are mean ± s.d. ( n = 3 cultures per strain or per condition; Statistical significance of AGE concentration of mutant strains is compared to AGE concentration of WT for the corresponding day/condition; *, P < 0.05; unpaired t -test; two-sided).

Article Snippet: 100 μL of protein extract, at concentrations ranging from 0.5 to 1.0 mg/mL, was added to an AGE conjugate-coated plate as part of an OxiSelect AGE Competitive ELISA Kit (Cell Biolabs).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Mutagenesis

Journal: Preventive Nutrition and Food Science

Article Title: NRF2 Activation Suppressed Methylglyoxal-Lysine Dimer-Induced Oxidative Stress and Inflammatory Paracrine Interaction between Macrophages and Adipocytes

doi: 10.3746/pnf.2025.30.5.450

Figure Lengend Snippet: Effects of MOLD with LPS on AGEs and RAGE production in RAW 264.7 cells. (A) Chemical structure of MOLD. (B) Cell proliferation analysis using the CCK-8 assay in RAW 264.7 cells. (C) Analysis of AGEs production using an ELISA kit in RAW 264.7 cell lysates. (D) AGEs and RAGE protein expression confirmed by Western blot analysis in RAW 264.7 cells. β-Actin was used as an internal control. (E) IHC staining of AGEs in RAW 264.7 macrophages incubated with or without MOLD for 24 h (×400 magnification). (F) IHC staining of RAGE in macrophages incubated with or without MOLD for 24 h in RAW 264.7 cells (×400 magnification). Each data point represents the mean±standard error of the mean for n=3 experiments. * P <0.05. MOLD, methylglyoxal-lysine dimer; LPS, lipopolysaccharide; AGEs, advanced glycation end products; RAGE, receptor for AGEs; CCK-8, Cell Counting Kit-8; ELISA, enzyme-linked immunosorbent assay; IHC, immunohistochemistry.

Article Snippet: Moreover, the cells were harvested to measure AGE concentration using the OxiSelect TM AGE Competitive ELISA Kit (Cell Biolabs Inc.) in accordance with the manufacturer’s instructions.

Techniques: CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control, Immunohistochemistry, Incubation, Cell Counting

Journal: Preventive Nutrition and Food Science

Article Title: NRF2 Activation Suppressed Methylglyoxal-Lysine Dimer-Induced Oxidative Stress and Inflammatory Paracrine Interaction between Macrophages and Adipocytes

doi: 10.3746/pnf.2025.30.5.450

Figure Lengend Snippet: Effects of MOLD with LPS on NO, PGE 2 production, and cytokine levels in RAW 264.7 cell culture media. (A) Nitrite production in RAW 264.7 cells. (B) iNOS protein expression in RAW 264.7 cells. (C) PGE 2 production in RAW 264.7 cells. (D) COX-2 protein expression in RAW 264.7 cells. (E) TNF-α levels measured by ELISA in RAW 264.7 cells. (F) IL-6 levels measured by ELISA in RAW 264.7 cells. (G) TNF-α protein expression in RAW 264.7 cells. β-Actin was used as an internal control. (H) IL-6 protein expression in RAW 264.7 cells. β-Actin was used as an internal control. Each data point represents the mean±standard error of the mean for n=3 experiments. * P <0.05. MOLD, methylglyoxal-lysine dimer; LPS, lipopolysaccharide; NO, nitric oxide; PGE 2 , prostaglandin E2; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase-2; TNF-α, tumor necrosis factor alpha; ELISA, enzyme-linked immunosorbent assay; IL-6, interleukin-6.

Article Snippet: Moreover, the cells were harvested to measure AGE concentration using the OxiSelect TM AGE Competitive ELISA Kit (Cell Biolabs Inc.) in accordance with the manufacturer’s instructions.

Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Preventive Nutrition and Food Science

Article Title: NRF2 Activation Suppressed Methylglyoxal-Lysine Dimer-Induced Oxidative Stress and Inflammatory Paracrine Interaction between Macrophages and Adipocytes

doi: 10.3746/pnf.2025.30.5.450

Figure Lengend Snippet: Effects of macrophage-derived CM on 3T3-L1 cells. (A) AGEs were analyzed using an ELISA kit in 3T3-L1 cell lysates. (B) AGEs and RAGE protein expression was analyzed by Western blot analysis in 3T3-L1 cells. β-Actin was used as an internal control. (C) ORO staining for the analysis of lipid accumulation in 3T3-L1 cells (×200 magnification). (D) Cell cycle analysis in 3T3-L1 cells. Cell populations at each stage were determined using the FCS Express 4 Flow Research Edition program. (E) c/EBPβ, PPARγ, and c/EBPα protein expression in 3T3-L1 cells. β-Actin was used as an internal control. (F) TNF-α and IL-6 protein expression in 3T3-L1 cells. β-Actin was used as an internal control. Each data point represents the mean±standard error of the mean for n=3 experiments. * P <0.05. CM, conditioned medium; AGEs, advanced glycation end products; ELISA, enzyme-linked immunosorbent assay; RAGE, receptor for AGEs; ORO, Oil Red O; TNF-α, tumor necrosis factor alpha; IL-6, interleukin-6.

Article Snippet: Moreover, the cells were harvested to measure AGE concentration using the OxiSelect TM AGE Competitive ELISA Kit (Cell Biolabs Inc.) in accordance with the manufacturer’s instructions.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control, Staining, Cell Cycle Assay

Journal: Heliyon

Article Title: Effect of nattokinase on the pathological conditions in streptozotocin induced diabetic rats

doi: 10.1016/j.heliyon.2024.e28835

Figure Lengend Snippet: Effect of NK on the elevation of AGE-albumin level in STZ-induced diabetic rats. The rats in the NK-L, NK-H, or control groups were fed low-NK diet (0.2 mg/g CE-2), high-NK diet (0.6 mg/g CE-2), and CE-2, respectively, from Day 0 to Day14 after STZ administration. Rats in the sham group (sham) were fed CE-2 for 14 days without STZ injection. As described in the Materials and Methods section, the circulating AGE-albumin levels in each group on day 14 measured using AGE-competitive ELISA kit. Data are expressed as mean ± S.E.M. # p < 0.05 versus sham (Student's t -test), * p < 0.05 versus control (Dunnett's test).

Article Snippet: OxiSelect™ Advanced Glycation End Product (AGE) Competitive ELISA Kit was obtained from Cell Biolabs (Danvers, MA, USA).

Techniques: Injection, Competitive ELISA